Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones Anthopleura dowii and Lebrunia neglecta
Open Access
- 23 December 2023
- journal article
- research article
- Published by MDPI AG in Marine Drugs
- Vol. 22 (1) , 12
Abstract
The bioprospecting of sea anemone tissues and secretions has revealed that they are natural libraries of polypeptides with diverse biological activities that can be utilized to develop of biotechnological tools with potential medical and industrial applications. This study conducted a proteomic analysis of crude venom extracts from Anthopleura dowii Verrill, 1869, and Lebrunia neglecta Duchassaing & Michelotti, 1860. The obtained data allowed us to identify 201 polypeptides, of which 39% were present in both extracts. Among the obtained sequences, hydrolase-type enzymes, oxidoreductases, transferases, heat shock proteins, adhesion proteins, and protease inhibitors, among others, were identified. Interaction analysis and functional annotation indicated that these proteins are primarily involved in endoplasmic reticulum metabolic processes such as carbon metabolism and protein processing. In addition, several proteins related to oxidative stress were identified, including superoxide dismutase, peroxiredoxins, thioredoxin, and glutathione oxidase. Our results provide novel information on the polypeptide composition of the crude venom extract from sea anemones, which can be utilized to develop molecules for therapeutic tools and industrial applications.Keywords
Funding Information
- Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT), Universidad Nacional Autónoma de México (IT200819, 203-2023, CONABIO-NE018)
- Instituto de Ciencias Aplicadas y Tecnología, UNAM (IT200819, 203-2023, CONABIO-NE018)
- Harte Research Institute for the Gulf of Mexico Studies (IT200819, 203-2023, CONABIO-NE018)
- Texas A&M University at Corpus Christi (IT200819, 203-2023, CONABIO-NE018)
- Comisión Nacional para el Conocimiento y Uso de la Biodiversidad (IT200819, 203-2023, CONABIO-NE018)
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