Stimulation of osteoblastic differentiation and inhibition of interleukin‐6 and nitric oxide in MC3T3‐E1 cells by pomegranate ethanol extract

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Abstract
In this experiment, we studied the effects of pomegranate fruit extract (PE) on the function of osteoblastic MC3T3‐E1 cells and the production of local factors in osteoblasts. PE (16∼250 µg/ml) significantly increased the growth of MC3T3‐E1 cells (P < 0.05). Moreover, PE (50 µg/ml) caused a significant elevation of alkaline phosphatase (ALP) activity and collagen content in the cells. We then examined the effect of PE on the TNF‐α‐induced production of interleukin‐6 (IL‐6) and nitric oxide (NO) in osteoblasts. Treatment with PE (10∼50 µg/ml) decreased the TNF‐α (10−10 M)‐induced production of IL‐6 and NO in osteoblasts. Copyright © 2008 John Wiley & Sons, Ltd.

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