A null allele of the pol IV second subunit impacts stature and reproductive development in Oryza sativa
- 22 June 2022
- journal article
- research article
- Published by Wiley in The Plant Journal
- Vol. 111 (3) , 748-755
Abstract
SUMMARY: All eukaryotes possess three DNA‐dependent RNA polymerases, Pols I–III, while land plants possess two additional polymerases, Pol IV and Pol V. Derived through duplication of Pol II subunits, Pol IV produces 24‐nt short interfering RNAs that interact with Pol V transcripts to target de novo DNA methylation and silence transcription of transposons. Members of the grass family encode additional duplicated subunits of Pol IV and V, raising questions regarding the function of each paralog. In this study, we identify a null allele of the putative Pol IV second subunit, NRPD2, and demonstrate that NRPD2 is the sole subunit functioning with NRPD1 in small RNA production and CHH methylation in leaves. Homozygous nrpd2 mutants have neither gametophytic defects nor embryo lethality, although adult plants are dwarf and sterile.Keywords
Funding Information
- Division of Molecular and Cellular Biosciences (1929678)
This publication has 59 references indexed in Scilit:
- Dual Binding of Chromomethylase Domains to H3K9me2-Containing Nucleosomes Directs DNA Methylation in PlantsCell, 2012
- DDR complex facilitates global association of RNA polymerase V to promoters and evolutionarily young transposonsNature Structural & Molecular Biology, 2012
- BEDTools: a flexible suite of utilities for comparing genomic featuresBioinformatics, 2010
- RNA polymerase V transcription guides ARGONAUTE4 to chromatinNature Genetics, 2009
- Subunit Compositions of the RNA-Silencing Enzymes Pol IV and Pol V Reveal Their Origins as Specialized Forms of RNA Polymerase IIMolecular Cell, 2009
- Noncoding Transcription by RNA Polymerase Pol IVb/Pol V Mediates Transcriptional Silencing of Overlapping and Adjacent GenesCell, 2008
- Sorting of Small RNAs into Arabidopsis Argonaute Complexes Is Directed by the 5′ Terminal NucleotideCell, 2008
- PolIVb influences RNA-directed DNA methylation independently of its role in siRNA biogenesisProceedings of the National Academy of Sciences, 2008
- Role of RNA polymerase IV in plant small RNA metabolismProceedings of the National Academy of Sciences, 2007
- T‐DNA insertional mutagenesis for functional genomics in riceThe Plant Journal, 2000