Nitrogenase from nifV mutants of Klebsiella pneumoniae contains an altered form of the iron-molybdenum cofactor
Authors:
- T R HawkesTHT R Hawkes
- P A McLeanPMP A McLean
- B E SmithBSB E Smith
- 1 January 1984
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 217 (1) , 317-321
Abstract
When the iron-molybdenum cofactor (FeMoco) was extracted from the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae and combined with the FeMoco-deficient MoFe protein from a nifB mutant, the resultant MoFe protein exhibited the NifV phenotype, i.e. in combination with wild-type Fe protein it exhibited poor N2-fixation activity and its H2-evolution activity was inhibited by CO. These data provide strong evidence that FeMoco contains the active site of nitrogenase. The metal contents and e.p.r. properties of FeMoco from wild-type and nifV mutants of K. pneumoniae are very similar.Keywords
This publication has 14 references indexed in Scilit:
- Nitrogenase of Klebsiella pneumoniae nifV mutants. Investigation of the novel carbon monoxide-sensitivity of hydrogen evolution by the mutant enzymeBiochemical Journal, 1983
- Requirement of nifV gene for production of wild-type nitrogenase enzyme in Klebsiella pneumoniaeNature, 1981
- Isolation of a molybdenum--iron cluster from nitrogenase.Proceedings of the National Academy of Sciences, 1981
- Large-scale purification of high activity Azotobacter vinelandii nitrogenaseBiochimica et Biophysica Acta (BBA) - Enzymology, 1980
- Structure and Function of NitrogenaseAnnual Review of Biochemistry, 1979
- Novel metal cluster in the iron-molybdenum cofactor of nitrogenase. Spectroscopic evidenceJournal of Biological Chemistry, 1978
- Isolation of an iron-molybdenum cofactor from nitrogenaseProceedings of the National Academy of Sciences, 1977
- Studies by electron paramagnetic resonance on the catalytic mechanism of nitrogenase of Klebsiella pneumoniaeBiochemical Journal, 1973
- Spin–spin interaction between molybdenum and one of the iron–sulphur systems of xanthine oxidase and its relevance to the enzymic mechanismBiochemical Journal, 1972
- Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteinsBiochemical Journal, 1972