Increased plasma levels of TGF‐beta1 and CD14+/CD105+ numbers in by‐pass patients correlate to increased CD105‐induced expression after plasma incubation

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Abstract
Objective: Compare the numbers of CD14+/CD105+, plasma levels of TGF‐beta1, apoptosis and induced CD105 expression in atherosclerotic patients (bypass surgery) and control ones (valvular patients). Methods: Blood was taken from our patients and CD34+/CD144+, CD34+/KDR+ and CD14+/CD105+ cells were assessed by flow cytometry (R=0.8908). Plasma was added to peripheral blood mononuclear cells. CD105 expression was assessed by Western blot (day 4). Apoptosis was measured as DNA fragmentation (day 7). Results: By‐pass patients had a higher number of CD14+/CD105+ (5.357 vs 2.829% lymphocytes, p=0.04; 87.13 vs 49.77 cells/mcL, p=0.0339) and a higher level of plasmatic TGF‐beta1 (72.01 vs 48.33, p=0.0033). When plasma from by‐pass and valvular was added to cell (day 7), valvular plasma triggered apoptosis (0.9998 vs 1.599, p=0.0078), an effect mimicked when plasma from by‐pass was coincubated with the TGF‐beta1 blockers SIS3 (0.9998 vs 1.624, p=0.0034) and SB‐431542 (0.9998 vs 1.802, p=0.0108). In Western blot, a trend was found towards higher CD105 expression with plasma from bypass (0.94 vs. 0.57, p=0.18). Conclusion: Plasma from by‐pass patients deregulates the effect of TGF‐beta1, which correlates to increased plasmatic TGF‐beta1 levels and CD14+/CD105+ cells and a trend towards higher in vitro expression of CD105. Disclosure: Funded by FISS PI041018 (Spanish Ministry of Health).