Determining the Genetic Difference of Some World Cotton Genotypes Using iPbs (Inter-Primer Binding Sequences) Retrotransposon Markers

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Abstract
This research was carried out with the aim of revealing the genetic differences of 30 cotton varieties from 14 countries using the iPBS-retrotransposon marker method. Genetic differences were determined by using iPBS marker method in PCR for 30 cotton varieties. According to the genetic distance matrix obtained in the NTSYS-pc 2.10e program, the closest genotypes were determined to be AzGR-11468 (Azerbaijan) and Maydos Yerlisi (India), and the similarity value between them was found to be 0.0318. The most distant genotypes were determined to be Hill Cotton 3 (Bangladesh) and Shazbaz (Pakistan), and the similarity value between them was found to be 0.7572. When the dendrogram generated based on the UPGMA method was examined.30 cotton varieties were divided into 2 clades. According to the PCA made in the JMP program, the graphic results and eigen values obtained on the two-dimensional plane also supported these results. The first 3 eigen values explained 85% of the total variance in the population. Overall results suggest that Hill Cotton 3 (Bangladesh) (Gossypium arboreum) and Shazbaz (Pakistan) (Gossypium hirsutum) cultivars, which are the most distant relatives among 30 cotton genotypes, can be used as breeding cultivars in future cotton breeding studies.
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  • This work was not supported by any agency.

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