Raman Spectroscopy and Improved Inception Network for Determination of FHB-Infected Wheat Kernels
Open Access
Abstract
Detection of infected kernels is important for Fusarium head blight (FHB) prevention and product quality assurance in wheat. In this study, Raman spectroscopy (RS) and deep learning networks were used for the determination of FHB-infected wheat kernels. First, the RS spectra of healthy, mild, and severe infection kernels were measured and spectral changes and band attribution were analyzed. Then, the Inception network was improved by residual and channel attention modules to develop the recognition models of FHB infection. The Inception–attention network produced the best determination with accuracies in training set, validation set, and prediction set of 97.13%, 91.49%, and 93.62%, among all models. The average feature map of the channel clarified the important information in feature extraction, itself required to clarify the decision-making strategy. Overall, RS and the Inception–attention network provide a noninvasive, rapid, and accurate determination of FHB-infected wheat kernels and are expected to be applied to other pathogens or diseases in various crops.Keywords
Funding Information
- Anhui Provincial Key Research and Development Plan (202004a06020032, 202104a06020025)
- National Natural Science Foundation of China (31971789, 32001421)
This publication has 43 references indexed in Scilit:
- Detecting Fusarium head blight in wheat kernels using hyperspectral imagingBiosystems Engineering, 2015
- Advanced methods of plant disease detection. A reviewAgronomy for Sustainable Development, 2014
- Surface-Enhanced Raman Spectroscopy Applied to Food SafetyAnnual Review of Food Science and Technology, 2013
- Raman spectroscopy: Recent advancements, techniques and applicationsVibrational Spectroscopy, 2011
- Detection of Fusarium damaged kernels in Canada Western Red Spring wheat using visible/near-infrared hyperspectral imaging and principal component analysisComputers and Electronics in Agriculture, 2011
- How to do successful gene expression analysis using real-time PCRMethods, 2010
- Plant Diseases, Pests and Food Security2010
- Proteins can convert to β‐sheet in single crystalsProtein Science, 2004
- New Technologies for Sensitive and Specific Routine Detection of Plant Pathogenic Bacteria2001
- Characteristics of the Microplate Method of Enzyme-Linked Immunosorbent Assay for the Detection of Plant VirusesJournal of General Virology, 1977