Ultracentrifugal isolation of serum chylomicron‐containing fractions with quantitation by infrared spectrometry and NCH elemental analysis

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Abstract
An ultracentrifugal method for isolating chylomicron‐containing fractions from serum by flotation, using either standard Spinco swinging‐bucket rotors or a specially fabricated swinging‐bucket rotor, is described. Lower limits of the Sf rates of the chylomicron fractions are evaluated using a computer technique to define lipoprotein flotation over a nonlinear NaCl density gradient. The latter is prepared by a special overlayering technique.Quantitation within a 9–50 μg region of mass assay is accomplished by both infrared spectrometry and elemental analysis for N, C and H. Results indicate that the chylomicron concentration in serum for a small population of nonfasting male adults ranges from approximately 0–50 mg %.

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