Stable Isotope Techniques for Assessing Labile Cu in Soils: Development of an L-Value Procedure, Its Application, and Reconciliation with E Values

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Abstract
Isotopic techniques have become a valuable tool for assessing the lability or potential availability of elements in soil. Until now, work on soil Cu has been limited to E-value methods where soil solution extracts are obtained by physical means due to the very short (12.4 h) half-life of the radio isotope 64Cu. However, a stable isotope method has recently been developed for determining soil Cu E values that utilizes enhancement of the 65Cu isotope in soil and measurement of the subsequent ratio with 63Cu. We have developed an L-value technique for soil Cu, where plants are used to sample the soil solution and therefore give a direct measure of the plant available Cu. The L-value technique developed was then compared, and found to be equivalent, with E values using equilibration periods up to and including the growth period of plants in the L-value method.

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