Simple Detection of Fluorescent Stained IgM in Sucrose Gradients: Demonstration of Virus-Specific IgM

Authors:
  • HSHerbert Schmitz
    Herbert Schmitz
  • CKChrista Maria Krainick-Riechert
    Christa Maria Krainick-Riechert
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Abstract
Serum samples were mixed with appropriate amounts of fluorescein isothio-cyanate (FITC) at pH 9, then centrifuged on sucrose gradients. After separation, the FITC-conjugated IgM antibodies could be removed from the gradient under direct visual control. Staining of the sera facilitated the isolation and detection of IgM as demonstrated for rubella IgM-containing sera.