Improved transformation of C3H10T1/2CL8 cells by direct- and indirect-acting carcinogens

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Abstract
Oncogenic transformation of C3H10T1/2CL8 cells was improved by treating the cells 5 days after seeding. Benzo[a]pyrene-induced transformation was increased 3.5-fold by this method, compared with treating the cells 1 day after seeding. N-Methyl-N′ -nitro-N-nitrosoguanidine, which does not transform asynchronous cultures of C3H10T1/2CL8 cells when administered 1 day after seeding, produced an average of 1 focus/dish, with 61% of the dishes exhibiting foci, when administered 5 days after seeding. Propane sultone and aflatoxin B1 also produced marked transformation responses when administered 5 days after seeding. However, 4-dimethylaminoazobenzene did not induce transformation when administered either 1 day or 5 days after seeding. With all chemicals examined, clonal cytotoxicity was reduced when they were administered 5 days after seeding. These results indicate the utility of this new procedure for the qualitative analysis of the transforming ability of chemicals.

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