cDNA clones encoding leucine‐zipper proteins which interact with G‐CSF gene promoter element 1‐binding protein
- 2 March 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 299 (1) , 36-38
Abstract
The gene expression of granulocyte colony‐stimulating factor (G‐CSF) is induced by lipopolysaccharide (LPS). GPE1, a cis‐controlling element of the G‐CSF gene, functions as an LPS‐responsive element. GPE1‐binding protein (GPE1‐BP), a leucine‐zipper protein, did not independently activate G‐CSF gene expression. Protein blot analysis with biotinylated GPE1‐BP revealed that there were nuclear proteins that interact specifically with GPE1‐BP. Three leucine‐zipper proteins were isolated from mouse cDNA expression libraries by this method: NF‐IL6, ATF4, and a novel ATF4‐related ATFx. The interactions of these proteins with GPE1‐BP may play key roles in G‐CSF gene expression.Keywords
This publication has 12 references indexed in Scilit:
- Three individual regulatory elements of the promoter positively activate the transcription of the murine gene encoding granulocyte colony-stimulating factorGene, 1991
- Molecular cloning of cDNA and a chromosomal gene encoding GPE1‐BP, a nuclear protein which binds to granulocyte colony‐stimulating factor promoter element 1FEBS Letters, 1991
- Ig/EBP-1: a ubiquitously expressed immunoglobulin enhancer binding protein that is similar to C/EBP and heterodimerizes with C/EBP.Genes & Development, 1990
- A nuclear factor for IL‐6 expression (NF‐IL6) is a member of a C/EBP family.The EMBO Journal, 1990
- Regulatory elements responsible for inducible expression of the granulocyte colony-stimulating factor gene in macrophages.Molecular and Cellular Biology, 1990
- [6] Use of T7 RNA polymerase to direct expression of cloned genes1990
- Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.Genes & Development, 1989
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding ProteinsScience, 1988
- Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDaAnalytical Biochemistry, 1987