SWI6 protein is required for transcription of the periodically expressed DNA synthesis genes in budding yeast
Authors:
- Noel F. LowndesNLNoel F. Lowndes
- Anthony L. JohnsonAJAnthony L. Johnson
- Linda BreedenLBLinda Breeden
- Leland H. JohnstonLJLeland H. Johnston
- 11 June 1992
- journal article
- Published by Springer Nature in Nature
- Vol. 357 (6378) , 505-508
Abstract
In budding yeast many genes are expressed under cell-cycle control in late G1. These include a large group of DNA synthesis genes, the HO gene involved in mating-type switching, CTS1 (chitinase) and also CLN1 and CLN2 (ref. 4) encoding G1 cyclins. Two factors, encoded by the SWI4 and SWI6 genes, are required for HO (ref. 5), CLN (refs 6, 7) and CTS1 (ref. 3) gene expression and, at least in the HO promoter, bind to CACGA4 upstream sequences (CCBs). This motif is not found upstream of the DNA synthesis genes, which instead have a hexamer element, ACGCGT1 (MCB), an MluI restriction site, that is recognized by a cell-cycle regulated transcription complex DSC1 (ref. 1). This MluI-activation system consisting of the MCBs and DSC1 is conserved in fission yeast where a DSC1-like complex controls the cdc22+ ribonucleotide reductase gene. The Schizosaccharomyces pombe cdc10+ gene encodes a component of DSC1 (ref. 10) and, significantly, this has homology with both the Swi4 and Swi6 proteins. Here we show that Swi6 is an essential component of DSC1 and that deletion of SWI6 impairs the cell-cycle regulation of the DNA synthesis genes, as well as CLN1 and CLN2. Thus Swi6 is the common factor in regulation of all the above genes and may therefore be responsible for the timing of their expression in late G1.Keywords
This publication has 21 references indexed in Scilit:
- Control of DNA synthesis genes in fission yeast by the cell-cycle gene cdclO+Nature, 1992
- Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast.Genes & Development, 1991
- Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcriptionCell, 1991
- The role of SWI4 and SWI6 in the activity of G1 cyclins in yeastCell, 1991
- Coordination of expression of DNA synthesis genes in budding yeast by a cell-cycle regulated trans factorNature, 1991
- G1-specific cyclins of S. cerevisiae: Cell cycle periodicity, regulation by mating pheromone, and association with the p34CDC28 protein kinaseCell, 1990
- The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcriptionNature, 1989
- Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of DrosophilaNature, 1987
- Cell cycle control of the yeast HO gene: Cis- and Trans-acting regulatorsCell, 1987
- Molecular analysis of a cell lineageNature, 1983