Subcellular localization of VIP1 is regulated by phosphorylation and 14‐3‐3 proteins

Authors:
Publisher website
Google Scholar
Abstract
Arabidopsis basic leucine zipper transcription factor VIRE2‐interacting protein 1 (VIP1) changes its localization from the cytosol to the nucleus when cells are subjected to mechanical or hypo‐osmotic stress, although the mechanism of this change is not known. In this study, we show that change in VIP1 subcellular localization is synchronized with a change in the VIP1 phosphorylation state that is induced by mechanical/hypo‐osmotic stress. VIP1 has three phosphorylatable serine residues in HXRXXS motifs, which are 14‐3‐3‐binding targets. Mutations of these residues results in the lack of 14‐3‐3 binding and prevents cytosolic localization of VIP1. These results suggest that dephosphorylation of VIP1 resulting from mechanical or hypo‐osmotic stress induces nuclear localization via 14‐3‐3 dissociation.

This publication has 37 references indexed in Scilit: