Multi-Modal Imaging with a Toolbox of Influenza AReporter Viruses
Authors:
- Vy TranVTVy Tran
- Daniel S. PooleDPDaniel S. Poole
- Justin J. JefferyJJJustin J. Jeffery
- Timothy P. SheahanTSTimothy P. Sheahan
- Donald CreechDCDonald Creech
- Aleksey YevtodiyenkoAYAleksey Yevtodiyenko
- Andrew J. PeatAPAndrew J. Peat
- Kevin P. FrancisKFKevin P. Francis
- Shihyun YouSYShihyun You
- Andrew MehleAndrew Mehle
Open Access
- 13 October 2015
- Vol. 7 (10) , 5319-5327
Abstract
Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography–computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.Keywords
Funding Information
- National Institute of General Medical Sciences (R00GM088484, T32 GM07215)
- American Lung Association (RG-310016)
- Shaw Scientist Award
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