Evaluation of the Asparagine Synthetase Level in Leukemia Cells by Monoclonal Antibodies
Authors:
- 1 October 2012
- journal article
- research article
- Published by SAGE Publications in Hybridoma
- Vol. 31 (5) , 325-332
Abstract
L-Asparaginase (ASNase) is important for the treatment of childhood acute lymphoblastic leukemia. ASNase sensitivity has been shown to correlate with the asparagine synthetase (ASNS) protein content in acute lymphoblastic leukemia cell lines. However, there have been few studies to determine ASNS protein levels in human leukemias, since no appropriate monoclonal antibody is available for such quantitative analysis. In this study, we report the generation of anti-ASNS monoclonal antibodies, which are applicable to flow cytometry and enzyme-linked immunosorbent assay. These monoclonal antibodies should provide a valuable tool for the quantification of ASNS protein level and estimation of ASNase-resistance in leukemia cells.Keywords
This publication has 22 references indexed in Scilit:
- Asparagine synthetase is a predictive biomarker ofl-asparaginase activity in ovarian cancer cell linesMolecular Cancer Therapeutics, 2008
- Asparagine Synthetase ChemotherapyAnnual Review of Biochemistry, 2006
- Expression levels of asparagine synthetase in blasts from children and adults with acute lymphoblastic leukaemiaBritish Journal of Haematology, 2006
- Upregulation of asparagine synthetase and cell cycle arrest in t(12;21)-positive ALLLeukemia, 2004
- Asparagine synthetase expression alone is sufficient to induce l-asparaginase resistance in MOLT-4 human leukaemia cellsBiochemical Journal, 2001
- Structural Basis for the Activity and Substrate Specificity of Erwinia chrysanthemi l-Asparaginase,Biochemistry, 2001
- Asparagine synthetase activity in paediatric acute leukaemias: AML‐M5 subtype shows lowest activityBritish Journal of Haematology, 2000
- A gene expression database for the molecular pharmacology of cancerNature Genetics, 2000
- Cell cycle arrest and apoptosis of leukemia cells induced by L-asparaginaseLeukemia, 1997
- Amino acid control of asparagine synthetase: relation to asparaginase resistance in human leukemia cellsAmerican Journal of Physiology-Cell Physiology, 1997