Primary structures of new ‘iso‐hirudins’
Authors:
- 11 September 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 255 (1) , 105-110
Abstract
Crude hirudin (12.7 U/μg), a complex mixture of polypeptides obtained from the leech, could be separated by microbore HPLC. A combination of amino acid analysis, N‐terminal microsequencing and chemical as well as enzymatic fragmentation made the primary sequence of the new isohirudins Ia‐IIIb' accessible. The biological activity determined in the thrombin inhibition test showed a comparable value for all of these compounds. The results presented address the question as to whether these isohirudins are true mutations from a family of genes or a family of leeches.Keywords
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