Ginsenoside Rd production from the major ginsenoside Rb1 by β-glucosidase from Thermus caldophilus
Authors:
- 8 November 2007
- journal article
- research article
- Published by Springer Nature in Biotechnology Letters
- Vol. 30 (4) , 713-716
Abstract
Under optimum conditions (pH 5, 75°C, and 0.2 U purified enzyme ml−1), 4 mg ginsenoside Rd was produced from 5 mg reagent-grade ginsenoside Rb1 in 5 ml after 30 min by β-glucosidase from Thermus caldophilus GK24. Using a ginseng root extract containing 1 mg ginsenoside Rb1 ml−1 and 3.2 mg additional ginsenosides ml−1, 1.23 mg ginsenoside Rd ml−1 was produced after 18 h; the concentrations of ginsenosides Rb1, Rb2, and Rc used for ginsenoside Rd production were 0.77, 0.17, and 0.19 mg ml−1, respectively.Keywords
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