An Effective Method of DNA Isolation from the Mature Leaves of Gossypium Species That Contain Large Amounts of Phenolic Terpenoids and Tannins

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Abstract
Purified and unstained nuclei were isolated from the leaves of several Gossypium species (diploid and tetraploid) by means of a citrate buffer (pH 5.0), Triton X-100 (5%), and a reducing sugar (1M glucose). DNA, previously unobtainable, was then extracted from the nuclei by conventional means. Comparisons of final DNA yield were made between three methods of purification; namely, the standardized ribonuclease procedure, hydroxyapatite chromatography and equilibrium density centrifugation in cesium chloride. The latter method produced the lowest, yet purest, yield of DNA for renaturation studies in Gossypium

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