Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging

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Abstract
By subdividing a charge-coupled device (CCD) array into subgroups using a digital micromirror device and offsetting exposure times, temporal pixel multiplexing allows simultaneous high-speed and high-resolution imaging using a single CCD. This imaging modality allows 250 Hz microscopic imaging of fast cellular responses with a 10-Hz 1.3 megapixel camera We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.

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