FIGURE 4 from Cancer-associated Fibroblast–specific Expression of the Matricellular Protein CCN1 Coordinates Neovascularization and Stroma Deposition in Melanoma Metastasis

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Abstract
CCN1-deficient stroma show disorganized collagen fibers. Digital pathology phenotypic quantification (PharmaNest, Inc) phenotypic fibrosis histologic heat chart (A) where each row represents a principal qFT, at the three phenotypic levels (collagen deposition, fibers morphometry, and fibrosis architecture). B, The Ph-FCS recapitulates all the qFTs for one sample, and quantifies the phenotype of fibrosis and its differences between CCN1-deficient (KO; n = 5, mean = 1.5, Std dev = 0.5) and wild-type (WT) mice (n = 6, mean = 4.6, Std dev = 0.9) groups (3.1 fold change, P < 0.001, Student t test). C, The collagen fiber morphometric scores recapitulates the differences at the morphometric level between KO (n = 5, mean = 1.6, Std dev = 0.5) and WT (n = 6, mean = 4.5, Std dev = 0.9) groups (2.9 fold change, P < 0.001, Student t test). D, Representative images with augmented digital pathology layers. “Entropy Texture” is one of the architectural phenotypes of fibrosis (39). KO, knockout.