A Comparison of Sample Preparation Techniques for the Determination of an Organo-phosphorous Pesticide from Water Using Reverse-Phase HPLC

Authors:
Publisher website
Google Scholar
Abstract
A methylene chloride liquid/liquid extraction and Sep-Pak C18 cartridge adsorption techniques were used to quantify the pesticide, Dursban, in contaminated environmental water samples. Results showed a large disparity between Dursban levels using these two techniques, due primarily to the presence of a large adsorbed fraction of pesticide. A Sample Clarification Kit was used to isolate the particulate fraction, which can subsequently be stripped of its adsorbed pesticide compliment by means of a methanol rinse. Lastly, the filtrate from the Sample Clarification Kit may be trace enriched on a Sep-Pak C18 cartridge to isolate the dissolved fraction of pesticide.