High performance liquid chromatography of natural products. I. Separation of cephalosporin C derivatives and cephalosporin antibiotics; isolation of cephalosporin C from fermentation broth.

Authors:
  • RMRoger D. Miller
    Roger D. Miller
  • NNNorbert Neuss
    Norbert Neuss
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Abstract
Microbonded propylamine silica with a solvent system containing acetic acid, methanol, acetonitrile and water (2:4:7.5:86.5) is suitable for an efficient separation of mixtures containing several closely related cephem derivatives. The same system with preparative columns was used for the isolation of cephalosporin C directly from the filtered broth of Cephalosporium acremonium fermentation.