Novel Method for Structure–Activity Relationship of Aptamer Sequences for Human Prostate Cancer

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Abstract
Prostate cancer (PCa) is one of the most common malignancies in men and seriously threatens men’s health. Developing aptamer probes for PCa cells is of great significance for early diagnosis and treatment of PCa. This paper reports a classification model for SELEX-based aptamers, which were obtained with PCa cell line PCa-3M-1E8 (highly metastatic tumor cell) as target cells and PCa cell line PCa-3M-2B4 (low metastatic tumor cell) as control cells. On the basis of the SELEX principle, 100 oligonucleotide sequences from the 3rd round of SELEX were defined as low affinity and specificity aptamers, and 100 sequences from the 11th round were set as high affinity and specificity aptamers. Seven molecular descriptors were used for the classification model, which were calculated from amino acid sequences translated from DNA aptamer sequences with DNAMAN software. The classification model based on binary logical regression analysis has prediction accuracies, sensitivity, and specificity of about 80% for both the training set and test set. Therefore, it is feasible to calculate molecular descriptors from amino acid sequence translated from DNA aptamer sequences and develop a classification model for PCa cell line PCa-3M-1E8.
Funding Information
  • Education Department of Hunan Province (16A047)
  • National Natural Science Foundation of China (21190044, 21190041)
  • State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University (2016013)

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