A ratiometric fluorescent nanoprobe for ultrafast imaging of peroxynitrite in living cells

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Abstract
For ratiometrically imaging peroxynitrite (ONOO−) in living cells, we devised and fabricated a novel fluorescent nanoprobe, NC-NP530/460, in this study. To achieve ratiometric fluorescence response towards ONOO−, NC-NP530/460 used 3-(2-benzothiazolyl) coumarin (Cou-Bz) as the internal reference and 1,8-naphthimide derivative (Naph-PN) as a fluorescent ONOO− probe. These compounds were incorporated into an amphiphilic block polymer called Pluronic F-127. In addition to an ultrafast response to ONOO−, NC-NP530/460 also showed great selectivity and sensitive detection (detection limit was 4.51 μM). It was important to note that NC-NP530/460 demonstrated solid performance for ONOO− fluorescence ratio imaging in living cells, highlighting its potential for ONOO−-related chemical biology research. Graphical abstract
Funding Information
  • Shandong Provincial Medical and Health Science and Technology Development Plan Program (202011001041)
  • National Natural Science Foundation of China (21971115)
  • Shandong traditional Chinese Medicine Science and Technology Development Plan Project (2021M219)

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