Vaccination with Alum-Precipitated Recombinant Ancylostoma-Secreted Protein 1 Protects Mice against Challenge Infections with Infective Hookworm (Ancylostoma caninum) Larvae

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Abstract
Ancylostoma-secreted protein 1 (ASP-1) is the major protein secreted by infective hookworm larvae (Ancylostoma caninum). The Escherichia coli-expressed recombinant protein was evaluated as a vaccine antigen in a mouse model of ancylostomiasis. A. caninum larvae migrate through mouse lungs, with maximal migration occurring 48- S4 h after oral infection. Quantitative larval recovery from the lungs at this time was used as an end point for vaccine evaluation. All mice developed antibodies to recombinant ASP-1 (rASP-1) after immunization and boosting with the alum-precipitated protein. The immunized mice had their worm burden reduced 79% (P < .0001) compared with controls. Immunization with rASP-1 in the presence of Corynebacterium parvum adjuvant also showed a vaccine effect (63% protection; P < .0001).The possibility that this protective effect resulted from delayed larval lung entry was excluded. rASP-l offers promise as a hookworm vaccine antigen.