Affinity chromatography of the cellulase system of Trichoderma koningii
Authors:
- G HalliwellGHG Halliwell
- M GriffinMGM Griffin
- 1 March 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 169 (3) , 713-715
Abstract
A procedure involving affinity chromatography on cellulose was developed for separating enzymic components of the cellulase complex. Cellobiase, carboxymethylcellulase, component C2 and cellobiohydrolase are adsorbed with increasing tenacity, and released, as highly purified components, as the ionic strength of the eluent is decreased.Keywords
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