Glucosinolate Pattern inIsatis tinctoriaandI. indigoticaSeeds

Authors:
  • MHMatthias Hamburger
    Matthias Hamburger
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Abstract
The glucosinolate patterns in seeds of five Isatis tinctoria and two Isatis indigotica accessions were assessed with a recently developed and validated LC-MS assay for direct analysis of glucosinolates without prior desulfatation. Glucosinolate peaks were identified with in-source fragmentation and detection of the sulfate anion (m/z = 97), and by MS/MS experiments. The glucosinolate patterns of the seeds showed characteristic differences compared to leaves. Glucoisatisin and epiglucoisatisin were diagnostic of seed samples. Qualitative and quantitative differences in glucosinolate patterns between both Isatis species were found for seed samples, enabling a differentiation of the two medicinal plants at the level of seed material. BPC:base peak chromatogram COX-2:cyclooxygenase-2 EIC:extracted ion chromatogram 5-LOX:5-lipoxygenase PLE:pressurized liquid extraction