Platinized carbon ultramicroelectrodes as glucose biosensors
Authors:
- Eugene R. ReynoldsEREugene R. Reynolds
- Alexander M. YacynychAYAlexander M. Yacynych
- 1 June 1993
- journal article
- research article
- Published by Wiley in Electroanalysis
- Vol. 5 (5-6) , 405-411
Abstract
Miniaturized glucose biosensors were constructed by all‐chemical means from disk‐shaped carbon ultramicroelectrodes (UMEs) (diameter = 8 μm). These ultramicrobiosensors (UMBs) are capable of direct measurement of glucose in the presence of interfering species. Carbon UMEs were partially platinized by the electrochemical reduction of a platinum (IV) complex. Platinization of the carbon UMEs gave an increased current response to hydrogen peroxide and allowed the modified electrode to be operated at a lower oxidative potential. Glucose oxidase (E.C. 1.1.3.4.) was immobilized either by glutaraldehyde cross‐linking or by direct covalent attachment to the electrode surface using carbodiimide. Response time (100% response) was 15–45 seconds. Response was linear over the range of 3–7 mM glucose. An electropolymerized film of poly(1,3‐diaminobenzene) was used to prevent interfering species.Keywords
This publication has 22 references indexed in Scilit:
- Microarray electrodes as biosensorsElectroanalysis, 1991
- Retention of enzyme by electropolymerized film: A new approach in developing a hypoxanthine biosensorBiotechnology and Bioengineering, 1991
- Electropolymerized films to prevent interferences and electrode fouling in biosensorsBiosensors and Bioelectronics, 1991
- Glucose fast-response amperometric sensor based on glucose oxidase immobilized in an electropolymerized poly(o-phenylenediamine) filmAnalytical Chemistry, 1990
- Electropolymerized 1,2-diaminobenzene as a means to prevent interferences and fouling and to stabilize immobilized enzyme in electrochemical biosensorsAnalytical Chemistry, 1990
- Electropolymerized Films in the Construction of Biosensors1989
- Surface control of platinized platinum as a transducer matrix for micro-enzyme electrodesJournal of Electroanalytical Chemistry and Interfacial Electrochemistry, 1988
- Enzyme microsensor for glucose with an electrochemically synthesized enzyme-polyaniline filmSensors and Actuators, 1988
- One Step Fabrication of Microbiosensor Prepared by the Codeposition of Enzyme and Platinum ParticlesAnalytical Letters, 1987
- Micro-Enzyme Electrode Prepared on Platinized PlatinumAnalytical Letters, 1987