Convenient genotyping of six myostatin mutations causing double‐muscling in cattle using a multiplex oligonucleotide ligation assay
Authors:
- W CoppietersWCW Coppieters
- L GrobetLGL Grobet
- M GeorgesMGM Georges
- A ValentiniAVA Valentini
- 1 December 2000
- journal article
- research article
- Published by Wiley in Animal Genetics
- Vol. 31 (6) , 396-399
Abstract
We herein describe a procedure that allows for simultaneous genotyping of six loss‐of‐function mutations in the bovine myostatin gene associated with the double‐muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.Keywords
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