Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses
- 2 February 2011
- journal article
- Published by Optica Publishing Group in Optics Express
- Vol. 19 (4) , 3130-3143
Abstract
We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording.Keywords
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