Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase
Open Access
- 4 December 2015
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 137 (49) , 15342-15345
Abstract
A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN reductase superfamily during redox cycling. The diversity of function within such a superfamily therefore has the potential to arise from rapid evolution, and its members should provide a convenient basis for developing new catalysts with an altered specificity of choice.Keywords
Funding Information
- National Institute of Diabetes and Digestive and Kidney Diseases (DK084186)
This publication has 38 references indexed in Scilit:
- Oxygen Reactivity in Flavoenzymes: Context MattersJournal of the American Chemical Society, 2011
- Efficient use and recycling of the micronutrient iodide in mammalsBiochimie, 2010
- A Conserved Active-site Threonine Is Important for Both Sugar and Flavin Oxidations of Pyranose 2-OxidaseJournal of Biological Chemistry, 2010
- A Mammalian Reductive Deiodinase has Broad Power to Dehalogenate Chlorinated and Brominated SubstratesJournal of the American Chemical Society, 2009
- Steady-State and Stopped-Flow Kinetic Studies of Three Escherichia coli NfsB Mutants with Enhanced Activity for the Prodrug CB1954Biochemistry, 2009
- Crystal Structure of Iodotyrosine Deiodinase, a Novel Flavoprotein Responsible for Iodide Salvage in Thyroid GlandsJournal of Biological Chemistry, 2009
- BluB cannibalizes flavin to form the lower ligand of vitamin B12Nature, 2007
- Enzymatic and Nonenzymatic Production of Free Radicals from the Carcinogens 4-Nitroquinoline N-Oxide and 4-Hydroxylaminoquinoline N-OxideChemical Research in Toxicology, 1999
- Isolation and morphological and cytological characterization of filamentous bacteria from bulking sludgeWater Research, 1990
- Über den Abbau von Dijodtyrosin im GewebeHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1950