G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila
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Open Access
- 26 July 2009
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 6 (8) , 603-605
Abstract
A Gal4-based system in Drosophila reports on gene expression at a given developmental stage combined with lineage information on expression at earlier developmental stages. We combined Gal4-UAS and the FLP recombinase–FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.Keywords
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