Deep-learning based 3D birefringence image generation using 2D multi-view holographic images

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Abstract
Refractive index stands as an inherent characteristic of a material, allowing non-invasive exploration of the three-dimensional (3D) interior of the material. Certain materials with different refractive indices produce a birefringence phenomenon in which incident light is split into two polarization components when it passes through the materials. Representative birefringent materials appear in calcite crystals, liquid crystals (LCs), biological tissues, silk fibers, polymer films, etc. If the internal 3D shape of these materials can be visually expressed through a non-invasive method, it can greatly contribute to the semiconductor, display industry, optical components and devices, and biomedical diagnosis. This paper introduces a novel approach employing deep learning to generate 3D birefringence images using multi-viewed holographic interference images. First, we acquired a set of multi-viewed holographic interference pattern images and a 3D volume image of birefringence directly from a polarizing DTT (dielectric tensor tomography)-based microscope system about each LC droplet sample. The proposed model was trained to generate the 3D volume images of birefringence using the two-dimensional (2D) interference pattern image set. Performance evaluations were conducted against the ground truth images obtained directly from the DTT microscopy. Visualization techniques were applied to describe the refractive index distribution in the generated 3D images of birefringence. The results show the proposed method’s efficiency in generating the 3D refractive index distribution from multi-viewed holographic interference images, presenting a novel data-driven alternative to traditional methods from the DTT devices.

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