Sororin loads to the synaptonemal complex central region independently of meiotic cohesin complexes

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Abstract
The distribution and regulation of the cohesin complexes have been extensively studied during mitosis. However, the dynamics of their different regulators in vertebrate meiosis is largely unknown. In this work, we have analyzed the distribution of the regulatory factor Sororin during male mouse meiosis. Sororin is detected at the central region of the synaptonemal complex during prophase I, in contrast with the previously reported localization of other cohesin components in the lateral elements. This localization of Sororin depends on the transverse filaments protein SYCP1, but not on meiosis‐specific cohesin subunits REC8 and SMC1β. By late prophase I, Sororin accumulates at centromeres and remains there up to anaphase II. The phosphatase activity of PP2A seems to be required for this accumulation. We hypothesize that Sororin function at the central region of the synaptonemal complex could be independent on meiotic cohesin complexes. In addition, we suggest that Sororin participates in the regulation of centromeric cohesion during meiosis in collaboration with SGO2‐PP2A. Synopsis This study analyses the distribution of Sororin, a regulatory factor of the cohesin complex, during male mouse meiosis. The results suggest different functions of Sororin at the synaptonemal complex and at the centromere. Sororin loads to the central region of the synaptonemal complex in a SYCP1‐dependent manner, but independently of cohesin complexes containing meiosis‐specific REC8 and SMC1β subunits. Sororin accumulates at the centromere by late prophase I and remains there until anaphase II. Sororin could participate in the regulation of centromeric cohesion during meiosis in collaboration with the SGO2‐PP2A complex.
Funding Information
  • Ministerio de Economía y Competitividad (SAF2011‐28842‐C02‐01)
  • Ministerio de Economía y Competitividad (BFU2014‐53681‐P)
  • Ministerio de Economía y Competitividad (SAF2011‐25252)
  • Ministerio de Economía y Competitividad (BFU2014‐59307‐R)
  • Ministerio de Economía y Competitividad (BFU2013‐48481‐R)
  • MEIONet (BFU2015‐71786‐REDT)
  • Junta de CyLeon
  • Fundación Mutua Madrileña (AP98712012)
  • Federación Española de Enfermedades Raras
  • Priority Program SPP 1384 of the German Science Foundation (Al 1090/2‐1)
  • Japan Society for the Promotion of Science (25891015)

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