Heterologous expression and secretion of Lactobacillus amylovorus α-amylase in Leuconostoc citreum
Authors:
- 18 July 2009
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 31 (11) , 1783-1788
Abstract
To develop a gene expression system for Leuconostoc genus, construction of expression vector and expression of a heterologus protein in Leuconostoc was performed. α-Amylase gene from Lactobacillus amylovorus was cloned into a Leuconostoc cloning vector, pLeuCM, with its own signal peptide. pLeuCMamy was introduced into Leuconostoc citreum CB2567 and a successful expression of α-amy gene was confirmed by enzyme activity assays. About 90% of α-amylase activity was detected in the culture broth, revealing most of expressed α-amylase was secreted out cells. The signal sequence of α-amy gene is a good candidate for the secretion of heterologous protein by using Leuconostoc host-vector system.Keywords
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