ANTIOXIDANT ACTIVITY TEST OF ANDROGRAPHOLIDE IN BITTER HERBS USING DPPH SCAVENGING

Authors:
  • NSNi Made Pitri Susanti
    Ni Made Pitri Susanti
  • NWNi Kadek Warditian
    Ni Kadek Warditian
  • IWI Made Agus Gelgel Wirasuta
    I Made Agus Gelgel Wirasuta
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Abstract
Bitter herbs (Sambiloto) have many pharmacological activities including antioxidant, antidiabetic, anticancer, antihyperlipidemic and anti-inflammation. Andrographolide is a diterpene compounds contained in bitter herbs. It is known that andrographolide compound responsible for the pharmacological activity of the bitter herbs. This study investigated DPPH free radical scavenging activity from andrographolide diterpene lactone. This study was initiated with the isolation of andrographolide compound from bitter herbs and then testing their DPPH free radical scavenging. The results suggested that andrographolide had IC50 value of 5.45 mg. This means andrographolide has 50% DPPH inhibition effect, i.e 5.45 mg.