Impedance based biosensor array for monitoring mammalian cell behavior
Authors:
- X. HuangXHX. Huang
- D.W. GreveDGD.W. Greve
- D.D. NguyenDND.D. Nguyen
- M.M. DomachMDM.M. Domach
- 8 July 2004
- conference paper
- Published by Institute of Electrical and Electronics Engineers (IEEE)
- Vol. 1, 304-309
Abstract
Functional genomic studies and drug candidate testing require tracking of cell activity with high throughput. Elec- trical impedance measurements on multiple electrodes are highly attractive in this application because of the potential for direct computer control. Impedance measurements over a wide range of frequency were performed on four sensing electrodes to study the mouse fibroblast cell behavior. We report the experimental observation of electrode imped- ance changes caused by cell growth and also the cell re- sponse to Protein Kinase Inhibitor (H-7) which affects cell attachments. The most notable signal is the peak magnitude of the normalized impedance change. The peak magnitude is related to the effective cell blockage which depends on the cell coverage, cell-substrate contacts and spaces. Dur- ing cell growth we observed the peak magnitude increased over the time and saturated. Furthermore, by characteriz- ing the peak magnitude we analyzed the effect of drug H-7 on the cell.Keywords
This publication has 8 references indexed in Scilit:
- Simulation of Microelectrode Impedance Changes Due to Cell GrowthIEEE Sensors Journal, 2004
- Light Microscopy Techniques for Live Cell ImagingScience, 2003
- Molecular diversity of cell-matrix adhesionsJournal of Cell Science, 1999
- Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structuresBiosensors and Bioelectronics, 1997
- Effect of protein kinase inhibitor H‐7 on the contractility, integrity, and membrane anchorage of the microfilament systemCell Motility, 1994
- Micromotion of mammalian cells measured electrically.Proceedings of the National Academy of Sciences, 1991
- Use of Electric Fields to Monitor the Dynamical Aspect of Cell Behavior in Tissue CultureIEEE Transactions on Biomedical Engineering, 1986
- Modern Electrochemistry1970