The effect of salt on the Michaelis Menten constant of the HIV‐1 protease correlates with the Hofmeister series
Authors:
- 25 March 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 280 (2) , 344-346
Abstract
The effect of different types of salt on the proteolytic activity of HIV‐1 protease was studied. At a similar ionic strength, the enzyme activity changed according to the salting out effect of the ions used (Hofmeister series). Kinetic studies showed that a stronger salting out effect of the ions rather than the higher ionic strength per se increased the affinity to the substrate (Km ) but in general did not alter the K cat value.Keywords
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