Design and Evaluation of pH-Sensitive Nanoformulation of Bergenin Isolated from Bergenia ciliata

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Abstract
The aim of the current study is extraction and isolation of bergenin from Bergenia ciliata and fabrication of pH-sensitive Eudragit® L100 (EL100) polymeric nanoparticles (NP) to tackle limitations of solubility. Bergenin-loaded EL100 nanoparticles (BN-NP) were fabricated via nanoprecipitation and an experimental design was conducted for optimization. A reverse phase-high performance liquid chromatography (RP-HPLC) method was developed for the quantitation of bergenin. The optimized nanoformulation was characterized by its particle size, morphology, loading capacity, entrapment efficiency, drug–excipient interaction and crystallinity. An in vitro assay was executed to gauge the release potential of pH-sensitive nanoformulation. The mean particle size, zeta potential and polydispersity index (PDI) of the optimized nanoparticles were observed to be 86.17 ± 2.1 nm, −32.33 ± 5.53 mV and 0.30 ± 0.03, respectively. The morphological analysis confirmed the spherical nature of the nanoparticles. Drug loading capacity and entrapment efficiency were calculated to be 16 ± 0.34% and 84 ± 1.3%, respectively. Fourier transform infrared spectroscopy (FTIR) studies unfolded that no interaction was present between the drug and the excipients in the nanoformulation. Crystallography studies revealed that the crystalline nature of bergenin was changed to amorphous and the nanoformulation was stable for up to 3 months at 40 °C. The present study confirms that bergenin isolation can be scaled up from abundantly growing B. ciliata. Moreover, it could also be delivered by entrapment in stimuli-responsive polymer, preventing the loss of drug in healthy tissues.

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