Influence of Triploid Musa spp. Genome Background and Exogenous Growth Regulators on In Vitro Regeneration in Plantains and Bananas

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Abstract
Bananas and plantains, belonging to the Musa genus, are important food crops that sustain the livelihoods of countless smallholder farmers globally. However, their production is hindered by various challenges, including abiotic and biotic stresses, climate change, and poor access to clean planting materials, which negatively impact their yields. Addressing these constraints is essential for improving production and ensuring food security. This study investigated the influence of triploid genome background and exogenous growth regulators on the regeneration of Musa cultivars [Gros Michel (AAA genome), Obino l’Ewai and Silk (AAB genome), and Poteau Naine (ABB genome)]. Shoot tip explants of the AAA, AAB, and ABB triploid genomes were cultured in Murashige and Skoog (MS) media supplemented with varying 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), or naphthaleneacetic acid (NAA) hormones. Shoot induction was successfully achieved within 21.50 ± 2.00 days, with AAA exhibiting the highest shoot induction frequencies ranging from 30.00 ± 1.57% to 100% and shoot numbers per explant ranging from 3.00 ± 0.50 to 8.80 ± 0.80, followed by the ABB genome ranging from 20.00 ± 3.45% to 100% and from 2.00 ± 0.55 to 5.60 ± 0.50 shoots, and the AAB genome ranging from 17.50 ± 5.01% to 100% and from 2.00 ± 0.04 to 6.60 ± 0.25 shoots, respectively, in media amended with 1.2 to 6.0 mg.L−1 BAP and 0.1 mg.L−1 IAA. The highest rooting rate of 100% was recorded in all three genomes in media containing 1.4 mg.L−1 IBA and 0.5 mg.L−1 IAA, with the AAA genome producing the maximum number of 14.8 roots per explant. The results indicate the positive influence of the AAA genome background on in vitro regeneration and its potential utilization for genomic editing transformation protocols
Funding Information
  • Department of Education Title III Part B HBCU (GR23000897, 60NANB23D215, 80NSSC22K0869)
  • NIST (GR23000897, 60NANB23D215, 80NSSC22K0869)
  • NASA (GR23000897, 60NANB23D215, 80NSSC22K0869)

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