An Optimized Protocol for Proximity Biotinylation in Confluent Epithelial Cell Cultures Using the Peroxidase APEX2
Open Access
- 1 September 2020
- journal article
- research article
- Published by Elsevier in STAR Protocols
- Vol. 1 (2) , 100074
Abstract
No abstract availableKeywords
Funding Information
- Agency for Science, Technology and Research
- Nanyang Technological University
This publication has 19 references indexed in Scilit:
- The Mammalian Crumbs Complex Defines a Distinct Polarity Domain Apical of Epithelial Tight JunctionsCurrent Biology, 2020
- Proximity labeling reveals novel interactomes in live Drosophila tissueDevelopment, 2019
- Determination of local chromatin interactions using a combined CRISPR and peroxidase APEX2 systemNucleic Acids Research, 2019
- Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress GranulesCell, 2018
- Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy PathwayMolecular Cell, 2017
- Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity LabelingCell, 2017
- Proximity-dependent biotin labelling in yeast using the engineered ascorbate peroxidase APEX2Biochemical Journal, 2016
- Proteomics of Primary Cilia by Proximity LabelingDevelopmental Cell, 2015
- Directed evolution of APEX2 for electron microscopy and proximity labelingNature Methods, 2014
- Proteomic Mapping of the Human Mitochondrial Intermembrane Space in Live Cells via Ratiometric APEX TaggingMolecular Cell, 2014