Identification of Hepatitis C Virus Transmission Using a Next-Generation Sequencing Approach

Authors:

1 April 2012

journal article
research article
Published by American Society for Microbiology in Journal of Clinical Microbiology

Vol. 50 (4) , 1461-1463
DOI:10.1128/jcm.00005-12
PMID:22301026
PMCID:PMC3318530

Abstract

Here, we describe a transmission event of hepatitis C virus (HCV) among injection drug users. Next-generation sequencing (NGS) was used to assess the intrahost viral genetic variation. Deep amplicon sequencing of HCV hypervariable region 1 allowed for a detailed analysis of the structure of the viral population. Establishment of the genetic relatedness between cases was accomplished by phylogenetic analysis. NGS is a powerful tool with applications in molecular epidemiology studies and outbreak investigations.

Keywords

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