Binding of actinomycin D to DNA revealed by DNase I footprinting
Authors:
- A.V. ScamrovASA.V. Scamrov
- R.SH. BeabealashvilliRBR.SH. Beabealashvilli
- 28 November 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 164 (1) , 97-101
Abstract
We have analyzed the specificity of the actinomycin D—DNA interaction. The ‘footprint’ method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X ≠ G and Y ≠ C in accordance with RNA elongation hindrance sites [1]. (iii) There is a positive cooperativity in binding of actinomycin D with DNA.Keywords
This publication has 9 references indexed in Scilit:
- Sequence‐specific inhibition of RNA elongation by actinomycin DFEBS Letters, 1983
- Stability of cloned promoter-containing fragmentsMolecular Genetics and Genomics, 1983
- Selective binding of actinomycin D and distamycin A to DNANucleic Acids Research, 1982
- Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II).Proceedings of the National Academy of Sciences, 1982
- [57] Sequencing end-labeled DNA with base-specific chemical cleavages1980
- Contacts between Escherichia coli RNA polymerase and a lac operon promoter.Proceedings of the National Academy of Sciences, 1978
- DNAase footprinting a simple method for the detection of protein-DNA binding specificityNucleic Acids Research, 1978
- Actinomycin. Chemistry and mechanism of actionChemical Reviews, 1974
- The Stereochemistry of Actinomycin Binding to DNA and Its Implications in Molecular BiologyProgress in Nucleic Acid Research and Molecular Biology, 1973