Expression of Interleukin-18 in Murine Contact Hypersensitivity

Authors:
  • KAKohji Aoyama
    Kohji Aoyama
  • SYSu Yu
    Su Yu
  • AKAtsushi Kitani
    Atsushi Kitani
  • HOHaruki Okamura
    Haruki Okamura
  • MKMasashi Kurimoto
    Masashi Kurimoto
  • TMTakami Matsuyama
    Takami Matsuyama
  • TMToshio Matsushita
    Toshio Matsushita
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Abstract
In this study, we made a mouse model for contact hypersensitivity (CH) using oxazolone as a contact allergen and examined the expression of interleukin-18 (IL-18) in the diseased skin sites at both mRNA and protein levels. In the kinetic study by semiquantitative RT-PCR, IL-18 mRNA was constitutively produced in normal murine skin but increased significantly at 12 h and peaked at 24 h in the ear skin of CH mice. A positive correlation was confirmed between the IL-18 mRNA signal and CH, as measured by mouse ear swelling response. Histologically, in situ hybridization showed that the IL-18 mRNA signal was weakly observed in the dermis but not the epidermis of normal skin, whereas the IL-18 mRNA signal was found intensively in the dermis, particularly in inflammatory cell areas. Using IL-18-specific antibody immunostaining, it was further found that IL-18 protein production had a histologic location similar to that of IL-18 mRNA in both normal and CH mice. The present study suggests that IL-18 may be implicated in the pathogenesis of murine CH.

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