Evaluation of Loop-Mediated Isothermal Amplification (LAMP) for Malaria Diagnosis in a Field Setting
Authors:
- Jeeraphat SirichaisinthopJSJeeraphat Sirichaisinthop
- Sureemas BuatesSureemas Buates
- Risa WatanabeRWRisa Watanabe
- Eun-Taek HanEHEun-Taek Han
- Wachira SuktawonjaroenponWSWachira Suktawonjaroenpon
- Somporn KrasaesubSKSomporn Krasaesub
- Satoru TakeoSTSatoru Takeo
- Takafumi TsuboiTTTakafumi Tsuboi
- Jetsumon SattabongkotJSJetsumon Sattabongkot
- 1 October 2011
- journal article
- research article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 85 (4) , 594-596
Abstract
We used the loop-mediated isothermal amplification (LAMP) method developed by our group for malaria diagnosis with genus-specific and species-specific primers for the four human malaria parasites at a field clinic in comparison with standard microscopy. Among 110 blood samples collected from the malaria clinic in Thailand, LAMP detected 59 of 60 samples positive by microscopy (sensitivity = 98.3%) and none of the 50 microscopy-negative samples (specificity = 100%). Negative predictive value (NPV) and positive predictive value (PPV) of LAMP were 98% and 100%, respectively. These results indicate that LAMP is an effective tool for malaria diagnosis at a field clinic in a field setting.Keywords
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