Up‐regulation of nitric oxide synthase by estradiol in human aorticendothelial cells
Authors:
- T NakakiTNT Nakaki
- T MarumoTMT Marumo
- H SuzukiHSH Suzuki
- R KatoRKR Kato
- T SarutaTST Saruta
- 6 March 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 360 (3) , 291-293
Abstract
We have examined the effects of sex hormones on calcium‐dependent NO production and protein levels of NO synthase in cultured human aortic endothelial cells, which were treated with various doses of 17β‐estradiol and testosterone for 8–48 h. Treatment with 17β‐estradiol enhanced calcium‐dependent NO production, but testosterone had exerted no effect. Western blot using monoclonal anti‐human endothelial NO synthase antibody clarified that increased NO production by 17β‐estradiol treatment was accompanied by increased NO synthase protein. Our results provide evidence that human endothelial NO synthase can be regulated by estrogens.Keywords
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