The use of a simple method to avoid cell shrinkage during SEM preparation
Authors:
- 1 February 1981
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 121 (2) , 185-189
Abstract
SUMMARY: In the course of preparing specimens for scanning electron microscopy, both glutaraldehyde and OsO4‐fixed cells exhibit a considerable shrinkage with a reduction of the mean cellular diameter of about 45% after critical point drying. However, if cells are successively treated with glutaraldehyde, OsO4, tannic acid and uranyl acetate solutions, cellular shrinkage of only 5% is observed.Keywords
This publication has 8 references indexed in Scilit:
- Volume changes during preparation of mouse embryonic tissue for scanning electron microscopyScanning, 1979
- Cell and organelle shrinkage during preparation for scanning electron microscopy: effects of fixation, dehydration and critical point dryingJournal of Microscopy, 1977
- Rapid Preparation of Uncoated Biological Specimens for Scanning Electron MicroscopyStain Technology, 1977
- Galloylglucoses of low molecular weight as mordant in electron microscopy. I. Procedure, and evidence for mordanting effect.Journal of Cell Biology, 1976
- The effects of preparative procedures for scanning electron microscopy on the size of isolated lymphocytesJournal of Anatomy, 1976
- Ligand-mediated osmium binding: Its application in coating biological specimens for scanning electron microscopyJournal of Ultrastructure Research, 1973
- Preparation of animal tissues for surface‐scanning electron microscopyJournal of Microscopy, 1969
- TECHNIQUES FOR THE PRESERVAATION OF THREE‐DIMENSIONAL STRUCTURE IN PREPARING SPECIMENS FOR THE ELECTRON MICROSCOPE*Transactions of the New York Academy of Sciences, 1951