This protocol allows for stereotaxic surgeries to administer unilateral or bilateral injections of small volumes of viral vectors or other suspensions into the mouse parenchyma. After using an anesthesia chamber to put the mouse to sleep, it is then placed and stabilized on the stereotaxic frame and is prepared for surgery. The mouse's bregma coordinates is used to calculate the appropriate coordinates needed to inject into specific brain regions.This protocol is optimized for differentbrain regions based on the coordinates listed in the guidelines.