Differentiation-dependent expression of human?-galactoside?2,6-sialyltransferase mRNA in colon carcinoma CaCo-2 cells

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Abstract
We have previously documented a dramatic elevation in the activity of α2,6-sialyltransferase towards Galβ1,4GlcNAc (EC 2.4.99.1) (α2,6ST) in CaCo-2 cells maintained in culture for several days after confluence to elicit a high degree of enterocytic differentiation phenotype. Northern analysis performed with a probe complementary to a region of human α2,6ST mRNA common to all known transcripts demonstrated that the expression of α2,6ST mRNA in CaCo-2 cells increased with the degree of differentiation. When probes complementary to 5′-untranslated exons (Y+Z or X) previously identified in transcripts isolated from human placenta and from several human lymphoblastoid cell lines were used, no hybridization signal with mRNA of CaCo-2 cells was found, as reported for the mRNA of hepatoma cell line HepG2 (Wang XC, Vertino A, Eddy RL, Byers MG, Jani-Sait SN, Shows TB, Lau JTY (1993)J Biol Chem 268: 4355–61). These results support the notion that the major α2,6ST transcript of CaCo-2 cells was the hepatoma isoform or a new one, so far unreported. Consistent with the differentiation-dependent increase in α2,6ST-mRNA expression, an elevation of the reactivity withSambucus nigra agglutinin of differentiated CaCo-2 cell-surface was observed, indicating an enhanced α2,6-sialylation of membrane glycoconjugates.

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